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Parasitology

Parasitological screening is performed to detect and identify ectoparasites and endoparasites. The basic method used is microscopy. Some parasites are identified by molecular biology (PCR, real-time PCR, sequencing), serological methods, or special histological staining of target tissues. Ectoparasites are detected with stereomicroscope and suspect parts of the skin are scraped and examined by microscopy. For detection of Endoparasites the samples have to be prepared by different methods (flotation of stool, total dissection of GIT, native smear/scrape of GIT mucosis- microscopy, filtration and flotation of gut content, urine sedimentation and urinary bladder mucosal scrape - rat only). For PCR detection, faeces and swab specimens from the skin are required.

Ektoparazites

Agents Method Host
Fleas stereomikroskopy M, R, Gp, H, Rb
Lice stereomikroskopy M, R, Gp, H, Rb
Mites scrape, microscopy, histology, PCR M, R, Gp, H, Rb

Endoparazites

Agents Method Host
Giardia spp. microscopy, ELISA, PCR M, R, Gp, H, Rb
Spironucleus muris microscopy, PCR M, R, H
Tritrichomonas spp. microscopy, PCR M, R, Gp, H
Entamoeba muris microscopy M, R, H
Entamoeba caviae microscopy Gp
Eimeria spp. microscopy M, R, Gp, Rb
Klosiella muris histopathology of kidneys M
Klosiella cobayae histopathology of kidneys Gp
Toxoplasma gondii IFA, histopathology M, R, Gp, H, Rb
Encephalitozoon cuniculi ELISA, IFA, histopathology M, R, Gp, H, Rb
Aspiculuris tetraptera microscopy, PCR M, R
Heterakis spp. microscopy R
Paraspidodera unicata microscopy Gp
Syphacia spp. microscopy, PCR M, R, H
Trichuris muris microscopy M, R
Capillaria hepatica histopathology, microscopy, gross pathology M, R, Gp, H, Rb
Trichosomoides crassicauda pathology (bladder), microscopy R
Graphidium strigosum pathology (stomach), , microscopy Rb
Hymenolepis spp. microscopy M, R, H
Development stages of parasites (Cestoda) pathology, microscopy M, R, Gp, H, Rb

Hosts: M - mouse, R - rat, Gp - Guinea pig, H - hamster, Rb – rabbit