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Parasitology

Parasitological screening is performed to detect and identify ectoparasites and endoparasites. The basic method used is microscopy. Some parasites are identified by molecular biology (PCR, real-time PCR, sequencing), serological methods, or special histological staining of target tissues. Ectoparasites are detected with stereomicroscope and suspect parts of the skin are scraped and examined by microscopy. For detection of Endoparasites the samples have to be prepared by different methods (flotation of stool, total dissection of GIT, native smear/scrape of GIT mucosis- microscopy, filtration and flotation of gut content, urine sedimentation and urinary bladder mucosal scrape - rat only). For PCR detection, faeces and swab specimens from the skin are required.

Ectoparasites

Agents Method Host
Myocoptes musculinus microscopy, PCR M
Myobia musculi microscopy, PCR M
Radfordia affinis microscopy, PCR M, R
Notoedres spp. microscopy R, H, Rb
Polyplax spp.  microscopy M, R
Chirodiscoides caviae microscopy Gp
Trixacarus caviae microscopy Gp
Gyropus ovalis microscopy Gp
Gliricola porcelli microscopy Gp
Psoroptes cuniculi microscopy Rb
Cheyletiella parasitovorax microscopy Rb
Listrophorus gibbus microscopy Rb
Haemodipsus spp. microscopy Rb
Ornithonyssus bacoti microscopy M, R, H

 

Endoparasites

Agens Metoda Host
Giardia spp. microscopy, ELISA, PCR M, R, Gp, H, Rb
Spironucleus muris microscopy, PCR M, R, H
Tritrichomonas spp. microscopy M, R, H, Gp
Entamoeba spp. microscopy M, R, H, Gp, Rb
Endolimax cavie microscopy Gp
Eimeria spp. microscopy, flotation M, R, Gp, Rb
Cryptosporidium spp. microscopy, flotation, histology M, H, Gp, Rb
Klossiella spp. histology of kidney M, Gp
Toxoplasma gondii IFA M, R, Gp, H, Rb
Encephalitozoon cuniculi ELISA, IFA, histology M, R, Gp, H, Rb
Syphacia spp. microscopy, flotation, PCR M, R, H
Aspiculuris spp. microscopy, flotation, PCR M, R
Passalurus ambiguus microscopy, flotation, PCR Rb
Paraspidodera unicata microscopy, flotation Gp
Trichuris muris microscopy, flotation M, R
Trichosomoides crassicauda microscopy, histology R
Rodentolepis spp. microscopy, flotation M, R, H
Hymenolepis spp. microscopy, flotation H

 

Abbreviations: M - Mouse, R - Rat, Gp - Guinea pig, H - Hamster, Rb – Rabbit