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Diagnostics

Diagnostics of undesirable agents in laboratory rodents and rabbits consists of methods in the fields of serology, bacteriology, parasitology and histology. Main serological methods used for detection of antibodies to viral agents, are ELISA (enzyme-linked immunosorbent assay) and IFA (immunofluorescence assay). Bacteriology is based on culture and followed by identification of bacteria with MALDI-TOF (matrix assisted laser desorption ionization – time of flight) or sequencing. Microscopy and coprology are the main methods for detection of ectoparasites and endoparasites. Histology is used when macroscopic lesions on organs and tissues are discovered at autopsy. For detection of DNA or RNA of undesirable organisms are also used molecular biology methods like PCR (polymerase chain reaction) and real-time PCR.