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Bacteriology

The culture on basic and selective-diagnostic culture media in aerobic, microaerophilic and anaerobic conditions is used for detection of pathogenic, opportunistic and usual microflora in laboratory rodents and rabbits. After microorganisms are isolated in pure culture, microscopic examination of colonies and identification of bacteria with MALDI-TOF or sequencing are performed. Molecular biology methods (PCR, real-time PCR), serological methods (ELISA, IFA, RPR, MAT) and histology are used for detection of some non-culturable bacteria. Identification of all pathogenic and opportunistic bacteria is accompanied with a professional commentary.

Bacteria

Agents Method Host
Bordetella bronchiseptica culture, MALDI-TOF R, Gp, Rb
Campylobacter jejuni culture, MALDI-TOF H
Filobacterium rodentium/Cilia-associated respiratory bacillus ELISA, IFA M, R, Rb
Chlamydophila psittaci IFA Gp
Citrobacter rodentium culture, MALDI-TOF, PCR M
Clostridium piliforme ELISA, IFA, PCR, histology M, R, H, Gp, Rb
Clostridium spp. culture, MALDI-TOF M, R, H, Gp, Rb
Corynebacterium bovis culture, MALDI-TOF M
Corynebacterium kutscheri culture, MALDI-TOF, PCR M, R, H, Gp
Haemophilus spp. culture, MALDI-TOF M, R, H, Gp, Rb
Escherichia coli culture, MALDI-TOF M, R, H, Gp, Rb
Helicobacter spp. PCR M, R, H
Helicobacter hepaticus PCR M, R
Helicobacter bilis PCR M, R
Helicobacter typhlonius PCR M, R
Helicobacter rodentium PCR M, R
Helicobacter muridarum PCR M, R
Klebsiella oxytoca culture, MALDI-TOF M, R, H, Gp, Rb
Klebsiella pneumoniae culture, MALDI-TOF M, R, H, Gp, Rb
Leptospira spp. MAT M, R, H, Gp, Rb
Mycoplasma pulmonis ELISA, IFA, culture, MALDI-TOF, PCR M, R
Mycoplasma arthitidis IFA R
Pasteurella multocida culture, MALDI-TOF Gp, Rb
Pasteurella spp. culture, MALDI-TOF M, R, H, Gp, Rb
Rodentibacter spp. (previously Pasteurella pneumotropica) culture, MALDI-TOF, PCR M, R, H, Gp
Proteus mirabilis culture, MALDI-TOF M, R, Gp, H, Rb
Proteus spp. culture, MALDI-TOF M, R, Gp, H, Rb
Pseudomonas aeruginosa culture, MALDI-TOF M, R, Gp, H, Rb
Pseudomonas spp. culture, MALDI-TOF M, R, Gp, H, Rb
Salmonella spp. culture, MALDI-TOF, PCR M, R, Gp, H, Rb
Staphylococcus aureus culture, MALDI-TOF, PCR M, R, Gp, H, Rb
Staphylococcus spp. culture, MALDI-TOF M, R, Gp, H, Rb
Streptobacillus moniliformis culture, MALDI-TOF, PCR M, R, Gp
Streptococcus pneumoniae culture, MALDI-TOF, PCR M, R, Gp, H, Rb
Beta-hemolytic streptococci group A, B, C, G culture, MALDI-TOF M, R, Gp, H, Rb
Treponema paraluiscuniculi RPR Rb
Yersinia pseudotuberculosis culture, MALDI-TOF M, R, H, Gp, Rb


Dermatophytes, microsporidia and pneumocystis

Agents Method Host
Trichophyton mentagrophytes culture, MALDI-TOF H, Gp, Rb
Microsporum spp. culture, MALDI-TOF Gp, Rb
Encephalitozoon cuniculi ELISA, IFA, histology Gp, Rb
Pneumocystis spp. IFA, PCR, histology M, R, Rb

 

Abbreviations: M - Mouse, R - Rat, Gp - Guinea pig, H - Hamster, Rb - Rabbit