The culture on basic and selective-diagnostic culture media in aerobic, microaerophilic and anaerobic conditions is used for detection of pathogenic, opportunistic and usual microflora in laboratory rodents and rabbits. After microorganisms in pure culture are isolated, the microscopic examination of colonies and identification of bacteria with MALDI-TOF or sequencing are performed. Molecular biology methods (PCR, real-time PCR), serological methods (ELISA, IFA, RPR, MAT) and histology are used for detection of some non-culturable bacteria. The result of identification all pathogenic and opportunistic bacteria is accompanied with a professional commentary.
Agents | Method | Host |
Bordetella bronchiseptica | culture, MALDI-TOF | R, Gp, Rb |
Campylobacter jejuni | culture, MALDI-TOF | H |
Filobacterium rodentium/Cilia-associated respiratory bacillus | ELISA, IFA | M, R, Rb |
Chlamydophila psittaci | IFA | Gp |
Citrobacter rodentium | culture, MALDI-TOF, PCR | M |
Clostridium piliforme | ELISA, IFA, PCR, histology | M, R, H, Gp, Rb |
Clostridium spp. | culture, MALDI-TOF | M, R, H, Gp, Rb |
Corynebacterium bovis | culture, MALDI-TOF | M |
Corynebacterium kutscheri | culture, MALDI-TOF, PCR | M, R, H, Gp |
Haemophilus spp. | culture, MALDI-TOF | M, R, H, Gp, Rb |
Escherichia coli | culture, MALDI-TOF | M, R, H, Gp, Rb |
Helicobacter spp. | PCR | M, R, H |
Helicobacter hepaticus | PCR | M, R |
Helicobacter bilis | PCR | M, R |
Helicobacter typhlonius | PCR | M, R |
Helicobacter rodentium | PCR | M, R |
Helicobacter muridarum | PCR | M, R |
Klebsiella oxytoca | culture, MALDI-TOF | M, R, H, Gp, Rb |
Klebsiella pneumoniae | culture, MALDI-TOF | M, R, H, Gp, Rb |
Leptospira spp. | MAT | M, R, H, Gp, Rb |
Mycoplasma pulmonis | ELISA, IFA, culture, MALDI-TOF, PCR | M, R |
Mycoplasma arthitidis | IFA | R |
Pasteurella multocida | culture, MALDI-TOF | Gp, Rb |
Pasteurella spp. | culture, MALDI-TOF | M, R, H, Gp, Rb |
Rodentibacter spp. (previously Pasteurella pneumotropica) | culture, MALDI-TOF, PCR | M, R, H, Gp |
Proteus mirabilis | culture, MALDI-TOF | M, R, Gp, H, Rb |
Proteus spp. | culture, MALDI-TOF | M, R, Gp, H, Rb |
Pseudomonas aeruginosa | culture, MALDI-TOF | M, R, Gp, H, Rb |
Pseudomonas spp. | culture, MALDI-TOF | M, R, Gp, H, Rb |
Salmonella spp. | culture, MALDI-TOF, PCR | M, R, Gp, H, Rb |
Staphylococcus aureus | culture, MALDI-TOF, PCR | M, R, Gp, H, Rb |
Staphylococcus spp. | culture, MALDI-TOF | M, R, Gp, H, Rb |
Streptobacillus moniliformis | culture, MALDI-TOF, PCR | M, R, Gp |
Streptococcus pneumoniae | culture, MALDI-TOF, PCR | M, R, Gp, H, Rb |
Beta-hemolytic streptococci group A, B, C, G | culture, MALDI-TOF | M, R, Gp, H, Rb |
Treponema paraluiscuniculi | RPR | Rb |
Yersinia pseudotuberculosis | culture, MALDI-TOF | M, R, H, Gp, Rb |
Agents | Method | Host |
Trichophyton mentagrophytes | culture, MALDI-TOF | H, Gp, Rb |
Microsporum spp. | culture, MALDI-TOF | Gp, Rb |
Encephalitozoon cuniculi | ELISA, IFA, histology | Gp, Rb |
Pneumocystis spp. | IFA, PCR, histology | M, R, Rb |
Abbreviations: M - Mouse, R - Rat, Gp - Guinea pig, H - Hamster, Rb - Rabbit