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Bacteriology

Various methods are used to detect pathogenic and opportunistic pathogenic microflora present in laboratory animals. The prevalence of bacteria is detected by culture on both basic and selective-diagnostic microbiological media in aerobic, microaerophilic and anaerobic conditions. Microscopic examination and biochemical characterization of colonies are performed after isolation of microorganisms in pure culture. Where appropriate, molecular biology methods (PCR, real-time PCR, sequencing) are used in diagnostics. Serological methods and special histological staining of target tissues are also included to identify certain bacteria. Identification of all opportunistic bacteria is accompanied by a professional commentary.

 

Infectious agents Method Host
Bordetella bronchiseptica culture R, Gp, Rb
Campylobacter jejuni culture H
Cilia associated respirátory bacillus ELISA, IFA M, R, Rb
Chlamydia psittacii IFA Gp
Citrobacter rodentium culture, PCR M
Clostridium piliforme ELISA, IFA, PCR, histopathology M, R, Gp, H, Rb
Clostridium spp. culture M, R, Gp, H, Rb
Corynebacterium bovis culture M
Corynebacterium kutscheri culture, PCR M, R, Gp, H
Haemophillus spp. culture M, R, Gp, H, Rb
Escherichia cole culture M, R, Gp, H, Rb
Helicobacter spp. PCR M, R, H
Helicobacter hepaticus PCR M, R
Helicobacter bilis PCR M, R
Helicobacter typhlonius PCR M, R
Helicobacter rodentium PCR M, R
Helicobacter muridarum PCR M, R
Klebsiella oxytoca culture M, R, Gp, H, Rb
Klebsiella pneumoniae culture M, R, Gp, H, Rb
Leptospira spp. serology (MAT) M, R, Gp, H, Rb
Mycoplasma pulmonis ELISA, IFA, kultivace, PCR M, R
Mycoplasma arthitidis IFA R
Pasteurella multocida culture R, Gp, Rb
Pasteurella pneumotropica culture, PCR M, R, Gp, H, Rb
Pasteurella spp. culture M, R, Gp, H, Rb
Proteus spp. culture M, R, Gp, H, Rb
Pseudomonas aeruginosa culture M, R, Gp, H, Rb
Pseudomonas spp. culture M, R, Gp, H, Rb
Salmonella spp. culture, PCR M, R, Gp, H, Rb
Staphylococcus aureus culture, PCR M, R, Gp, H, Rb
Staphylococcus spp. culture M, R, Gp, H, Rb
Streptobacillus moniliformis culture, PCR M, R, Gp
Streptococcus pneumoniae culture, PCR M, R, Gp, H, Rb
Streptococci β haemolytické/skupiny A, B, C, G culture, PCR M, R, Gp, H, Rb
Treponema cuniculi culture(RPR) Rb
Yersinia pseudotuberculosis culture M, R, Gp, H, Rb

 Mykology

Infectious agents Method Host
Kvasinky mikroskopie, culture M, R, Gp, H, Rb
Dermatofyta mikroskopie, culture M, R, Gp, H, Rb
Pneumocystis spp. PCR, Histopathology M, R

Hosts: M - mouse, R - rat, Gp - Guinea pig, H - hamster, Rb – rabbit