Parasitology

Parasitological screening is done for detection and identification of Ectoparasites and Endoparasites. The basic method for detection of parasites is microscopy but some parasites are identified by the serological methods or histology of target tissue. Ectoparasites are detected with stereomicroscope and suspect parts of skin are scraped and examined by microscopy. For detection of Endoparasites the samples have to be prepared by different method (flotation of stool, total dissection of GIT, native smear/scrape of GIT mucosis- microscopy, filtration and flotation of gut content, urine sedimentation and urinary bladder mucosal scrape - rat only).

Agents Method Natural Host
ECTOPARASITES
Fleas stereomicroscopy M, R, Gp, H, Rb
Lice stereomicroscopy M, R, Gp, H, Rb
Mites scrape, microscopy, histology M, R, Gp, H, Rb
ENDOPARASITES
Giardia sp. microscopy, ELISA M, R, Gp, H, Rb
Spironucleus muris microscopy M, R, H
Tritrichomonas sp. microscopy M, R, Gp, H
Entamoeba muris microscopy M, R, H
Entamoeba caviae microscopy Gp
Eimeria sp. microscopy M, R, Gp, Rb
Klosiella muris histology kidney M
Klosiella cobayae histology kidney Gp
Toxoplasma gondii IFA, histology M, R, Gp, H, Rb
Encephalitozoon cuniculi ELISA, IFA, histology M, R, Gp, H, Rb
Aspiculuris tetraptera microscopy M
Heterakis sp. microscopy R
Paraspidodera uncinata microscopy Gp
Syphacia sp. microscopy M, R, H
Trichuris muris microscopy M, R
Capillaria hepatica gross pathology, microscopy, histology M, R, Gp, H, Rb
Trichosomoides crassicauda pathology (urinary bladder), microscopy R
Graphidium strigosum gross pathology (stomach), microscopy Rb
Hymenolepis sp. microscopy M, R, H
Developmental stages of parasites (mainly class Cestoda) pathology, microscopy M, R, Gp, H, Rb as secondary host

M - mouse, R - rat, Gp - Guinea pig, H - hamster, Rb - rabbit