Bacteriology

The different methods are used for detection of present pathogenic and opportunistic microflora at laboratory animals. The prevalence of bacteria is detected by classical culture on usual and specific bacteriological media in aerobic, microaerophilic or anaerobic conditions. The following isolation of microorganism in pure culture, microscopy and biochemical characteristics are performed. Where necessary the molecular methods (Partial sequencing 16S rDNA with BLAST analysis and PCR amplification of genomic DNA) are used. For identification of some bacteria also serological methods and special histological staining are involved.

The identification of all oportunistic bacteria with professional comments are a matter of course.

Agents Method Natural Host
Bordetella bronchiseptica Culture R, Gp, Rb
Campylobacter jejuni Culture H
Cilia associated respiratory bacillus ELISA, IFA, Histology M, R, Rb
Chlamydia psittacii IFA Gp
Citrobacter rodentium Culture M
Clostridium piliforme PCR, ELISA, IFA, Pathology, Histology M, R, Gp, H, Rb
Clostridium spp. Culture M, R, Gp, H, Rb
Corynebacterium bovis Culture M
Corynebacterium kutscheri (murium) Culture M, R, Gp, H
Haemophilus spp. Culture M, R, Gp, H, Rb
Escherichia coli Culture M, R, Gp, H, Rb
Helicobacter spp. PCR M, R, H
Helicobacter hepaticus PCR M, R
Helicobacter bilis PCR M, R
Helicobacter muridarum PCR M, R
Helicobacter typhlonicus PCR M, R
Klebsiella oxytoca Culture M, R, Gp, H, Rb
Klebsiella pneumoniae Culture M, R, Gp, H, Rb
Leptospira spp. Serology (MAT) M, R, Gp, H, Rb
Mycoplasma pulmonis ELISA, IFA, Culture M, R
Mycoplasma arthritidis IFA R
Pasteurella multocida Culture R, Gp, Rb
Pasteurella pneumotropica Culture M, R, Gp, H, Rb
Pasteurella spp. Culture M, R, Gp, H, Rb
Proteus spp. Culture M, R, Gp, H, Rb
Pseudomonas aeruginosa Culture M, R, Gp, H, Rb
Pseudomonas spp. Culture M, R, Gp, H, Rb
Salmonella spp. Culture M, R, Gp, H, Rb
Staphylococcus aureus Culture M, R, Gp, H, Rb
Staphylococcus spp. Culture M, R, Gp, H, Rb
Streptobacillus moniliformis Culture M, R, Gp
Streptococcus pneumoniae Culture M, R, Gp, H, Rb
Streptococci β haemolytic / groupA,B,C,G Culture M, R, Gp, H, Rb
Treponema cuniculi Serology (RPR) Rb
Yersinia pseudotuberculosis Culture M, R, Gp, H, Rb

Mycology

Agents Method Natural Host
Yeast Microscopy, Culture M, R, Gp, H, Rb
Dermatophyta Microscopy, Culture M, R, Gp, H, Rb
Pneumocystis sp. PCR, Histology (special staining) M, R

M - mouse, R - rat, Gp - guinea pig, H - hamster, Rb - rabbit